MDH CLONES

To support MDH CURE projects a diverse set of wild-type MDH genes have been codon-optimized for expression in bacteria and cloned into an IPTG inducible His-tag expression vector.  All MDH 6X His Tags are cloned into the C-terminus of MDH.  Where indicated a TEV cleavage site has between the 6XHis tag and the coding region of MDH. Amino acid sequence numbering was used for creating each clone.  Some numbering may not be the same as other databases. See each MDH Protein Clone Datasheet for specific information.  Sequence verified mutants (wgMDH and other clones) are also available.  See below to review the entire list of wild-type clones available for MCC Faculty.

Upon request an aliquot of plasmid DNA, or a streaked agar plate can be shipped after a Material Transfer Agreement has been completed .  Contact Joseph Provost (josephprovost@sandiego.edu) for all wild-type clones and Jessica Bell (jessicabell@sandiego.edu) for watermelon mutants.   

Clones and mutants are available directly from Addgene