Videos

This page is a collection of all videos posted throughout the MCC website

Introduction to MDH CUREs Community (NSF Showcase)

Background on MDH CURE Research Projects

MDH CURES: Human MDH PPI & PTM Review

MDH CURES: Allostery, Loop, Structure…

MDH CURES: MDH CS Metabolon

MDH CURES:Alt Approach to MDH-HIS Purification

MDH CURES: PTM Phosphorylation

MDH CURES: PTM Ribosylation

Protocols, Assays and Techniques

MDH Assay Tricks, Tips and Hacks

Recombinant Purification PT 1 Plasmids, Strains and Transformation

Recombinant Purification Pt 2 Culture Lysis and Induction

Recombinant Purification Pt 3 Protein Purification

Graphing Kinetics Data on Excel: A tutorial

TBA

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How to use SnapGene to design SDM Primers for NEBQ5

TBA

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Virtual Workshops

2023 Protein Society CUREs Presentation

2023 MCC Workshop Day 2 - Techniques

2023 MCC Virtual Cohort Meeting - Creating your First MDH CURE

Tips and help for creating your MDHRE

Intro and helpful hints of Hypothesis Design

MDH CURES for Intro Courses

Bioinformatics and Computational MCC Videos

Part I: Introduction to teaching an undergraduate biochemistry laboratory as a CURE

Part II: Preparing a Protein Structure and Computational Approaches

Part III: Structure Refinement - RefineD

Part IV: Structure Validation - MolProbity

Part V: Creating Multimeric Proteins – HawkDock, PyMol

Part VI: Probing Protein Conformation & Local Environment – H++

Part VIII: Mapping Surface Properties using Fragment Mapping with SwissDock

Part IX: Exploring Protein Interactions with Other Proteins or Small Molecules

Part X: Examples of Binding Studies with Malate Dehydrogenase

MDH Journal Club & MDH Science Workshops

Journal Club: MDH Mechanism of Catalysis

Journal Club: Kinetic Mechanism

NMR Applications for MDH CURE

MDH Science: MDH Phosphorylation

MDH Science: Tools to Study MDH

MDH Science: Review of Structure, Allostery and Kinetics

MDH/MCC Publication Videos

BCA Assay for Assaying MDH (link to paper)

An alternative method to measure MDH activity using a spec in the vis wavelength range

Coming Soon

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The Role of the Flexible Loop (Paper Link)

Coming soon

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