Expression & Purification

Background Information

• Bacterial Strains for Protein Expression

• Basic Background on Bacterial Culture

• Background on Bacterial Strains for Expression (AddGene)

• Recombinant MDH Storage Instructions

• BioRad Profinity NI Charged

Protocols

• Bacterial Culture and Antibiotic Preparation

• Making LB Plates

• Streaking Bacteria

• Bacterial Expression of MDH Clones

• Simple Protocol for GOI Expression in E. coli Culture

• Bacterial Lysis for MDH Purification

• His-Tag/Ni-Agarose Purification of MDH

• Quick Bradford

• Dialysis

• Storage of Purified MDH Protein

Tutorials/ Videos

• MDH Purification: DNA, Bacterial Strains and Transformation

• MDH Purification: Culture, Expression and Lysis

• MDH Purification: Ni-Agarose Chromatography

Teaching Files

• Detailed handout on Expression, Lysis and Purification (see Provost for ppt)

• Short PPT on Purification

Budget Saver Ideas

Something missing or do you have something to add? Email Josephprovost@sandiego.edu